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Image Search Results
Journal:
Article Title: Lactoferrin acts as an alarmin to promote the recruitment and activation of antigen-presenting cells and antigen-specific immune responses
doi:
Figure Lengend Snippet: A) Monocyte migration was assayed using 48-well micro-chemotaxis chambers with the addition of monocytes (106/ml) and TLF in the upper and lower chambers, respectively. After 2 h of incubation, the membrane was scraped, fixed, and stained. Migrated monocytes were counted using Bioquant Life Science software. Results shown are the average +SD of seven experiments. *=p<0.01. B) Dose-response of monocyte migration to TLF. One experiment out of three is shown. C) Checkerboard analysis. Monocytes were allowed to migrate in the presence (+) of 1 μg/ml of TLF in the lower chamber or in both the upper and lower chambers. The results shown are the average (mean+SD) of three experiments. D) Effect of pertussis toxin (PTx). Monocytes were pretreated with 100 ng/ml PTx for 1 h at 37°C prior to utilization in the migration assay. 100ng/ml of CCL2 was used as a positive control for monocyte migration and to confirm the effect of PTx. The data show the average (mean+SD) of 4 experiments. *p<0.01; **p<0.05.
Article Snippet: In the 48-well chemotaxis assay, experiments were run in triplicates and migrated cells were fixed, stained and counted using
Techniques: Migration, Chemotaxis Assay, Incubation, Staining, Software, Positive Control